Table 2 Technological overview of organ-on-a-chip models of colorectal cancer.
In vitro platform | Cancer cell type | Other biological components | Material | Chip configuration | Fabrication technique | Media exchange method | Ref |
|---|---|---|---|---|---|---|---|
Vascularized micro-organs | HCT116, SW620, SW480 | ECs, matrix and stromal cells | PDMS | 2-layered channels | Soft lithography | Direct exchange | [77] |
Organotypic tumor spheroids | MC38, CT26, Primary cells from human and mouse (Organotypic Tumor Spheroids) | Organotypic Tumor Spheroids contains immune cells and stromal cells | PDMS | 3-layered channels | Soft lithography | Perfusion | [66] |
Colorectal tumor chip | HCT116 | HCoMEC | PDMS | 3-layered channels | Soft lithography | Perfusion | [86] |
CRC-on-chip | Caco2, C2BBe1, HCT116, HT29, CRC organoid | HUVEC and human primary fibroblasts | PDMS | 2-layered channels | Soft lithography | Perfusion | [60] |
Vascularized micro-tumors | HCT116, SW480 | ECs, matrix and fibroblasts | PDMS | 3-layered channels | Soft lithography | Direct exchange | [78] |
Tumor-on-chip | SW620 | Spheroids within Matrigel | Polycarbonate | 2-layered channels | Micromachining | Perfusion | [61] |
