Fig. 1: MLKL depletion protected livers against IR injury.

WT mice were subjected to liver IR model or sham procedure. Liver tissues and primary hepatocytes were collected at 6 h post reperfusion. A Western blot analysis of MLKL and GAPDH in liver tissues. B Immunofluorescence staining of DAPI (blue) and MLKL (red) in liver tissues. C Western blot analysis of MLKL and GAPDH in primary hepatocytes. WT and MLKL KO mice were subjected to liver IR model or sham procedure. Liver tissues, blood samples were collected at 6 h post reperfusion. D, E Serum levels of ALT and AST. F H&E‐staining of liver sections; Suzuki scores based on H&E. n = 5 mice/group. All results represent at least two independent experiments. Values were expressed as mean ± SD. *p < 0.05.