Fig. 2: MLKL depletion attenuated inflammatory liver IR injury by suppressing macrophage STING activation. | Cell Death Discovery

Fig. 2: MLKL depletion attenuated inflammatory liver IR injury by suppressing macrophage STING activation.

From: MLKL deficiency attenuated hepatocyte oxidative DNA damage by activating mitophagy to suppress macrophage cGAS-STING signaling during liver ischemia and reperfusion injury

Fig. 2

WT and MLKL KO mice were subjected to liver IR model or sham procedure. Liver tissues, blood samples and intrahepatic macrophages were collected at 6 h post reperfusion. A Gene expression of TNFα, IL-1β, IL-6 and IL-10 in liver tissues measured by qRT-PCR. B Expression of cGAS, TBK1, P-TBK1, and GAPDH in isolated intrahepatic macrophages measured by Western blot. C Immunofluorescence staining of DAPI (blue), F4/80 (red) and STING (green) in livers. WT and KO mice were pretreated with DMXAA (10 mg/kg) or PBS (Control) 3 h prior to ischemia. Mice were sacrificed 6 h poster reperfusion. D H&E‐staining of liver sections. E Serum levels of ALT and AST. F Gene expressions of TNFα, IL-1β, IL-6 and IL-10 in livers measured by qRT‐PCR. n = 5 mice/group. All results represent at least two independent experiments. Values were expressed as mean ± SD. *p < 0.05.

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