Fig. 3: MLKL deficiency inhibited macrophage STING activation by reducing hepatocyte oxidative DNA damage. | Cell Death Discovery

Fig. 3: MLKL deficiency inhibited macrophage STING activation by reducing hepatocyte oxidative DNA damage.

From: MLKL deficiency attenuated hepatocyte oxidative DNA damage by activating mitophagy to suppress macrophage cGAS-STING signaling during liver ischemia and reperfusion injury

Fig. 3

WT and MLKL KO mice were subjected to liver IR model or sham procedure. Liver tissues, blood samples and intrahepatic macrophages were collected at 6 h post reperfusion. A Immunofluorescence staining of DAPI (blue), 8-OHdG (red) in livers. B Serum levels of 8-OHdG. Mice were pretreated with anti-8-OHdG antibody (10 mg/kg) at 3 h prior to ischemia and sacrificed at 6 h post reperfusion. C Immunofluorescence staining of DAPI (blue), F4/80 (red) and STING (green) in livers. D Western blot analysis of cGAS, TBK1, P-TBK1, and GAPDH in isolated macrophages. n = 5 mice/group. All results represent at least two independent experiments. Values were expressed as mean ± SD. *p < 0.05.

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