Fig. 4: MK801 rescued LPS-increased permeability in HUVECs.

A Immunostaining images of F-actin labeled with TRITC Phalloidin and B analysis in HUVECs induced by LPS and treated with MK801; the peripheral bands of F-actin and intercellular gaps appeared between adjacent cells. C Vascular Permeability Assay showed the relative intensity of FITC-Dextran in HUVECs induced by LPS and treated with MK801. Representative of western blotting images (D) and analysis (E) of expression of ZO-1, Occludin, and Cx43 in LPS-exposed HUVECs in the presence or absence of different concentrations of MK801. (Data are presented as mean ± SEM, ***P < 0.001, **P < 0.01, *P < 0.05 vs. Control; ###P < 0.001, ##P < 0.01, #P < 0.05 vs. LPS).