Fig. 5: The relationship between TLR4 and NMDARs. | Cell Death Discovery

Fig. 5: The relationship between TLR4 and NMDARs.

From: NMDARs antagonist MK801 suppresses LPS-induced apoptosis and mitochondrial dysfunction by regulating subunits of NMDARs via the CaM/CaMKII/ERK pathway

Fig. 5

A Flow cytometry images and B analysis of FITC-conjugated LPS-treated HUVECs in the presence or absence of indicated doses of MK-801. C MTT assay showed relative cell viability in NMDA- or LPS-treated HUVECs in the presence or absence of MK801 or TAK242. D MTT assay showed relative cell viability in control siRNA or TLR4 siRNA transfected HUVECs treated with LPS or MK801. E Co-IP detection showed the interaction of NMDARs subunits, NMDAζ1, NMDAε1, NMDAε2, NMDAε3, and NMDAε4, with TLR4 induced by LPS in HUVECs. F The western blotting image showed the expression of NMDAζ1, NMDAε1, NMDAε2, and NMDAε4 treated with LPS in the presence or absence of different concentrations of MK801 in HUVECs. (Data are presented as mean ± SEM, ***P < 0.001, **P < 0.01 vs. Control or control siRNA; ##P < 0.01 vs. LPS or control siRNA + LPS; &P < 0.05 vs. NMDA; $P < 0.05 vs. TLR4 siRNA).

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