Fig. 6: Overexpression or suppression of KLF10 affected the ability of proliferation, repair and lumen-formation of renal tubular cells. | Cell Death Discovery

Fig. 6: Overexpression or suppression of KLF10 affected the ability of proliferation, repair and lumen-formation of renal tubular cells.

From: Downregulation of KLF10 contributes to the regeneration of survived renal tubular cells in cisplatin-induced acute kidney injury via ZBTB7A-KLF10-PTEN axis

Fig. 6

A, B Efficient overexpression of KLF10 was confirmed by immunoblot analysis and relative mRNA levels. C Representative immunoblot analysis of PCNA and CCND1 in KLF10 overexpressed group and control group. ACTIN served as the standard. D The cell viability of KLF10 overexpressed group and control group were measured by CCK-8 assay. E The ability to repair was measured by wound healing assay. F, G Representative fluorescence image of KLF10 overexpressed group and control group (2D/3D cultured). H Distribution of relative volume of tubule model was calculated and analyzed by Fiji. I Fluorescence images of 3D tubular cisplatin-induced AKI model (75 μM) for 9 h with or without overexpression of KLF10 reflecting the injury degree (LM and GFP) and proliferative activity (Fucci-overall, Merge and Fucci). J Relative mRNA levels of KLF10 in siRNA transfected group and control group were examined. K Representative immunoblot analysis of KLF10 and PCNA in siRNA transfected group and control group. ACTIN served as the standard. L Representative fluorescence image of Fucci in siRNA transfected group and control group. *p < 0.05, **p < 0.01, ***p < 0.001 vs control group at the same experimental conditions.

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