Fig. 1: pyr RNAi knock down impairs clearance of apoptotic cells by embryonic glia.
From: Drosophila FGFR/Htl signaling shapes embryonic glia to phagocytose apoptotic neurons
A-D” Single panes of the embryonic CNS at stage 16; ventral view. Bar, 20 µm. A-A”, C-C” Control embryos (gcmGal4/+;repoGal4,cytGFP/+) and B-B”, D-D” embryos expressing pyr RNAi in glia (gcmGal4/+;repoGal4,cytGFP/UASpyrRNAi) stained with anti-GFP (A, A”, B, B”, green) or anti-SIMU (C, C”, D, D”, green) and anti-Dcp-1 (red) antibodies. E Mean total volume of apoptotic particles within CNS sections and F percentages of apoptotic cell volume not engulfed by glia ± SEM, n = 9 (control), n = 8 (pyr RNAi). Statistical significance was analyzed employing Student’s t test, **p < 0.01, *p < 0.05. Note the significant increase in volume of apoptotic cells and higher percentage of non-engulfed particles in embryos expressing pyrRNAi in glia.
