Fig. 1: Metabolic analysis and IP3R1 expression of C57BL/6 mice on chow diet (CD) or high-fat diet (HFD). | Cell Death Discovery

Fig. 1: Metabolic analysis and IP3R1 expression of C57BL/6 mice on chow diet (CD) or high-fat diet (HFD).

From: Lack of adipocyte IP3R1 reduces diet-induced obesity and greatly improves whole-body glucose homeostasis

Fig. 1

A Body weight of mice maintained on CD or HFD for 8 wk (n = 15). B Tissue weight (eWAT, iWAT, TA and GAS) percentage of body weight (n = 6-7). C Representative HE-stained sections of iWAT and eWAT from mice fed CD or HFD (n = 6-7). Scale bar = 50 μm. D GTT (2 g/kg glucose, i.p.) (n = 10). E ITT (1 U/kg insulin, i.p.) (n = 10). F, G Blood glucose and plasma insulin levels under fasting and fed states (n = 9-10 for fasting state, n = 6 for fed state). H Relative mRNA expression levels of Ip3r1 in adipose tissues (iWAT and eWAT) and skeletal muscles (TA and GAS) (n = 6). I–L Western blot analysis of p-IP3R1 (Y353) and IP3R1 in TA and GAS lysates. Quantification of p-IP3R1 (Y353)/IP3R1 and IP3R1 was determined by ImageJ software (n = 6). Data were shown as means ± SEM. *P < 0.05, **P < 0.01. A–C, F–L: two-tailed unpaired Student’s t test; D and E: two-way ANOVA followed by Bonferroni’s post hoc test.

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