Fig. 2: Let-7b-5p targets HK2. | Cell Death Discovery

Fig. 2: Let-7b-5p targets HK2.

From: Let-7b-5p inhibits breast cancer cell growth and metastasis via repression of hexokinase 2-mediated aerobic glycolysis

Fig. 2

A, B Western blot for HK2 protein expression in indicates BC cells after transfection with let-7b-5p mimic or NC (A) or let-7b-5p inhibitor or scramble (B) (n = 3, mean ± SD). Histograms under western blot show let-7b-5p expression by RT-qPCR (n = 3, mean ± SD). C RT-qPCR assay of HK2 mRNA expression level in indicated BC cells after transfection with let-7b-5p mimic/inhibitor (n = 3, mean ± SD). D Dual-luciferase reporter assay of the indicated BC cells after transfection with wild-type or mutated HK2 reporter plus let-7b-5p mimic (n = 3, mean ± SD). The top panel shows the putative binding sites between HK2 and let-7b-5p. **p < 0.01 versus corresponding control.

Back to article page