Fig. 3: Let-7b-5p dampens BC cell growth, migration, and invasion via repression of HK2 expression. | Cell Death Discovery

Fig. 3: Let-7b-5p dampens BC cell growth, migration, and invasion via repression of HK2 expression.

From: Let-7b-5p inhibits breast cancer cell growth and metastasis via repression of hexokinase 2-mediated aerobic glycolysis

Fig. 3

A Growth curve was analyzed by CCK-8 Kit after MDA-MB-231 cells were transfected with NC, let-7b-5p mimic, or let-7b-5p mimic plus HK2 plasmid (n = 3, mean ± SD). Western blot and RT-qPCR showed HK2 and let-7b-5p expression, respectively. B Colony formation analysis of MDA-MB-231 cells after the transfection as in (A). Histograms display the colony number (n = 3, mean ± SD). C, D Scratch test (C) and transwell assay (D) of MDA-MB-231 cells after the transfection as in (A). Histograms display relative cell migration or invasion (n = 3, mean ± SD). E, F Control or HK2 shRNA MDA-MB-231 cells with the transfection of let-7b-5p inhibitor or scramble were analyzed as in (A) and (B) (n = 3, mean ± SD). G, H Scratch test (G) and transwell assay (H) of control or HK2 shRNA MDA-MB-231 cells with the transfection as in (E, F) (n = 3, mean ± SD). Scale bar, 100 μm. *p < 0.05, **p < 0.01 versus corresponding control.

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