Fig. 6: Let-7b-5p/HK2 axis modulates glycolysis, tumor proliferation, and metastasis in mice.

A, B Control or HK2 shRNA MDA-MB-231 cells were treated with anti-let-7b-5p (antagomiR-let-7b-5p) or scramble (antagomiR-NC) and injected into nude mice. Tumor size was detected at indicated times, and a proliferation curve was drew (n = 7, mean ± SD). C Lactic acid concentration of representative tumors from (A) was assayed (n = 3, mean ± SD). D Western blot of HK2 expression in representative tumors from (A). E Representative bioluminescence images were collected from lung metastasis models by injecting indicated MDA-MB-231 cells into the tail vein of nude mice (n = 5). The bioluminescence signal is presented by an overlaid false-color image with the signal intensity indicated by the scale. F Representative photographs of lungs from E and H&E staining of lung tissue sections. The scatter diagram showed the number of metastatic nodules.