Fig. 1: STING and its downstream effectors expression are excessively increased in macrophages of human patients with DW. | Cell Death Discovery

Fig. 1: STING and its downstream effectors expression are excessively increased in macrophages of human patients with DW.

From: High glucose-induced STING activation inhibits diabetic wound healing through promoting M1 polarization of macrophages

Fig. 1

A Sections of NC (upper row), NDW (middle row), and DW (lower row) were stained with H&E (Columns 1 and 2 on the left) or for IL-1β expression (Columns 3 and 4 on the left) and CD68 expression (right 2 columns). B Skin and wound lysates were examined for IL- 1β and CD68 using western blot analysis. Blots are quantified using bar graphs. C Sections of NC (upper row), NDW (middle row), and DW (lower row) were stained with co-localization of macrophages and STING using laser confocal microscopy. Anti-CD68 antibody labeled Macrophages (red), anti-STING antibody labeled STING (green) and DAPI labeled nucleus (blue). D Skin and wound lysates were examined for cGAS, STING, and inflammatory signaling using western blot analysis. Blots are quantified using bar graphs. For all bar graphs, Data were represented as mean ± SD (n = 9). *P < 0.05, **P < 0.01, and ***P < .001, NC vs NDW, NC vs DW, NDW vs DW.

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