Fig. 6: Immunoneutralization of NPCT increased hippocampal neuronal death in rat RSE model. | Cell Death Discovery

Fig. 6: Immunoneutralization of NPCT increased hippocampal neuronal death in rat RSE model.

From: Aminoprocalcitonin protects against hippocampal neuronal death via preserving oxidative phosphorylation in refractory status epilepticus

Fig. 6

A Schematic showing the procedure of peripheral NPCT immunoneutralization. B Success rate of modeling in the three groups. C Behavioral latency to RSE onset in animals receiving intraperitoneal anti-NPCT. One-way ANOVA test. n = 15–18 rats in each group. D Heatmap showing the percentages of daily death of animals receiving intraperitoneal anti-NPCT within 14 d after RSE onset. E Survival curves comparing the mortality within 14 d after RSE onset. Log-rank test. n = 15–18 rats in each group. **P < 0.01, compared with RSE + Veh group. F, G The proportion of rats with acute death and latent death respectively. Chi-square test. n = 15–18 rats in each group. H–K FjC staining showing degenerating neurons in CA1, CA3, and DG after intraperitoneal anti-NPCT administration in RSE. One-way ANOVA with Turkey’s post hoc analyses. n = 3 rats in each group. ***P < 0.001, compared with control group; # #P < 0.01, ###P < 0.001, compared with RSE + Veh group; &&P < 0.01, &&&P < 0.001, compared with RSE + NRI group. L–O FjC staining showing degenerating neurons in CA1, CA3, and DG after intracerebroventricular anti-NPCT administration in RSE. One-way ANOVA with Tukey’s post hoc analyses. n = 6 rats in each group. ***P < 0.001, compared with control group; #P < 0.05, ###P < 0.001, compared with RSE + Veh group; &P < 0.05, &&&P < 0.001, compared with RSE + NRI group. All data except (B, F, G) are presented as mean ± SEM. Data in (B, F, G) are presented as percentages with the numbers in columns representing the animal number. NRI nonimmune rabbit IgG.

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