Fig. 2: GSDME-dependent pyroptosis signaling pathway is activated in DN rats.
From: GSDME-dependent pyroptosis signaling pathway in diabetic nephropathy
A Western blotting analysis of caspase-3, p17, GSDME, GSDME-N, caspase-1, p20, IL-1β, and mIL-1β in kidney cortex of the DN and Ctrl group at 12, 16, and 20 weeks (n = 3 per group). B–E Quantification analysis of p17, GSDME-N, p20 and mIL-1β in DN and Ctrl group (n = 3 per group). F The mIL-1β concentrations in serum at 12, 16, and 20 weeks (n = 5 per group). G Immunohistochemistry staining of caspase-3 p17, caspase-1 p20, GSDME and mIL-1β in Ctrl and DN rats at 12, 16 and 20 weeks (scale bar: 50 µm). H TUNEL staining was performed in the kidney cortex for each group (scale bar: 100 µm). *P < 0.05, **P < 0.01, ***P < 0.001 vs. Ctrl rats.
