Fig. 1: Expression of Sun2 protein is elevated at fibrotic area of muscles in Z24−/− mice.
From: Nuclear softening mediated by Sun2 suppression delays mechanical stress-induced cellular senescence
A Gastrocnemius (GM) skeletal muscle tissues were harvested from 5-month-old wild-type (WT) and Z24−/− mice. Immunofluorescent staining of Sun2 (red) and Collagen type I (Col I)(green) showed increased number of Sun2-high cells in Z24−/− muscle; also, Sun2-high cells seem to be prone to appear at Collagen type I-rich fibrotic area, which is supposed to have higher mechanical stiffness. White arrows mark the position of Sun2-high cells at Collage type I-rich area, and orange arrows mark the position of Sun2-low cells. Yellow arrow heads mark the area with high collagen deposition (stiffer area). B Statistics of the number of Sun2-high cells in muscles. C Trichrome staining of WT and Z24−/− muscle tissues to compare the amount of fibrotic tissues. D Statistics of the number of SA-β-Gal+ cells in WT and Z24−/− muscles. N ≥ 6 for data analysis, with at least three biological replicates and two technical replicates. * indicates p < 0.05.
