Fig. 3: Immune response in DSD and TGCT samples.
From: Genomic instability in individuals with sex determination defects and germ cell cancer
Heat maps (a) represents a GO selection of immune response proteins from the entire proteome mass spectrometry data of leukocytes from individuals with DSD. Blue labeled the upregulated and green labeled the downregulated proteins in DSD-GCT, red labeled proteins are significantly upregulated in CAIS group. qRT-PCR data of innate immune specific gene expression in leukocytes of IFNβ (b), ISG15 (c) for DSD group and IFNβ (d), ISG56 (e) for TGCT group. Immune fluorescence analysis of TGCT gonadal tissue biopsy for STING and OCT4 proteins (f; n(TGCT) = 523; n(control)=871; arrow points to OCT4-negative non-GCT cell that has STING in the nucleus, in contrast to other OCT4-positive cells with STING exclusively concentrated in the cytoplasm) or dsDNA (h; n(TGCT) = 2140; n(control)=783; arrow points to colocalization of dsDNA and STING in the cytoplasm) and the quantification (g, i). Unpaired t test was used for statistical analysis, p values are indicated. Scale 0,4 μm (f, h). n-number of analyzed cells. Cyt cytoplasm; nucl nucleus.
