Fig. 4: MDH1 promoted ROS production by mediating the conversion of α-KG to 2-HG. | Cell Death Discovery

Fig. 4: MDH1 promoted ROS production by mediating the conversion of α-KG to 2-HG.

From: α-KG inhibits tumor growth of diffuse large B-cell lymphoma by inducing ROS and TP53-mediated ferroptosis

Fig. 4

A The mRNA levels of MDH1 in DLBCL samples from the GEO database (GSE56315). B, C OCI-LY1 and OCI-LY10 cells were treated with different agents for 24 h: (1) 0.1% DMSO; (2) 15 mM DM-αKG; (3) 10 μM MDH1-IN-2 pre-treatment followed by 15 mM DM-αKG. DCFDA assay was applied to detect intracellular ROS levels, represented by flow cytometry plots (B) and relative fluorescence quantification (C). D OCI-LY1 and OCI-LY10 cells were treated with 0.1% DMSO or DM-αKG (5, 10, 15, 20, 25 mM) for 24 h. Supernatant 2-HG levels were examined by 2-HG assay. E OCI-LY1 and OCI-LY10 cells were treated with different agents for 24 h: (1) 0.1% DMSO; (2) 15 mM DM-αKG; (3) 10 μM MDH1-IN-2 pre-treatment followed by 15 mM DM-αKG. Supernatant 2-HG levels of drug-treated cells were detected by 2-HG assay. All data were presented as means ± SD of 3 independent experiments. The data were analyzed by two-tailed t test in (A, C, E), or one-way ANOVA followed by Dunnett’s multiple comparison test in (D). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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