Fig. 6: α-KG promoted oxidative DNA damage and TP53 expression to mediate ferroptosis. | Cell Death Discovery

Fig. 6: α-KG promoted oxidative DNA damage and TP53 expression to mediate ferroptosis.

From: α-KG inhibits tumor growth of diffuse large B-cell lymphoma by inducing ROS and TP53-mediated ferroptosis

Fig. 6

A Heatmap analysis of ferroptosis pathway in RNA-seq. B Correlation analysis of TP53 and DNA damage-related genes in DLBCL samples from the GEO database (GSE56315). C–E OCI-LY1 and OCI-LY10 cells were treated with 0.1% DMSO or 15 mM DM-αKG for 24 h. DNA fragments were examined by neutral comet assay, which were stained with DAPI (upper panel). DNA damage was determined by tail moments calculation, where 50 cells were counted in each individual experiment (lower panel) (C). Protein levels of ATM, p-ATM, p-H2AX, and TP53 were examined by immunoblot analysis, where wild-type cells without drug treatment were used as the negative control (Blank) (D). Oxidative DNA damage was determined by the 8-OHdG detection assay (E). Immunoblot images in (D) were the representation of 3 independent experiments. All data were presented as means ± SD of 3 independent experiments. The data were analyzed by two-tailed t test in (C) and (E). *P < 0.05, ****P < 0.0001.

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