Fig. 6: The miR-146a-5p regulated TRAF6-IRAK1 complex and the IKK-dependent NF-κB pathway. | Cell Death Discovery

Fig. 6: The miR-146a-5p regulated TRAF6-IRAK1 complex and the IKK-dependent NF-κB pathway.

From: MiR-146a-5p deficiency in extracellular vesicles of glioma-associated macrophages promotes epithelial-mesenchymal transition through the NF-κB signaling pathway

Fig. 6

Wound healing (A, C) and transwell assays (B, D) were used to estimate the invasive and migratory abilities of U87MGmiR-146a-5p, U87MGov-TRAF6/IRAK1, and U87MGsh-TRAF6/IRAK1 cells compared with the corresponding controls. Scale bar = 200 μm. E, F Western blotting analysis as used to evaluate the levels of E-cadherin, N-cadherin, MMP-2, and vimentin in U87MGmiR-146a-5p, U87MGov-TRAF6/IRAK1, and U87MGsh-TRAF6/IRAK1 cells and their corresponding controls. G, H Western blotting was conducted to measure the expression of IKKγ, phosphorylated-IKKγ (p-IKKγ), IKBα, p-IKBα, p65, and p-p65 in U87MGmiR-146a-5p, U87MGov-TRAF6/IRAK1, and U87MGsh-TRAF6/IRAK1 cells and their corresponding controls. The ratios of p-IKKγ/IKKγ, p-IKBα/IKBα, and p-p65/p65 were calculated. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001.

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