Table 1 sgRNA sequences and primers used for validation of gene knockout.
From: Biallelic FBXW7 knockout induces AKAP8-mediated DNA damage in neighbouring wildtype cells
sgRNA sequences for CRISPR-Cas9 | |
|---|---|
Gene target | sgRNA sequence |
FBXW7 | GUCCGCUGUGUUCAAUAUGA |
AAVS1 (PPP1R12C) | GGGGCCACUAGGGACAGGAU |
AKAP8 | CUCGAACGGCUGGAAGCGGA |
Sequencing primers to validate gene knockout | ||
|---|---|---|
Gene target | Forward primer | Reverse primer |
FBXW7 | TCTGCAGAGTTGTTAGCGGT | TGGACTGTACTGGATCAGCAA |
AAVS1 (PPP1R12C) | GGTCCGAGAGCTCAGCTAGT | GGCTCCATCGTAAGCAAACC |
AKAP8 | AGTGTTCCAGGGAGGGTGTT | GGGTCTCGGCATTCACTGTA |
