Table 2 Antibodies and concentrations of antibodies used for immunofluorescence.
From: Biallelic FBXW7 knockout induces AKAP8-mediated DNA damage in neighbouring wildtype cells
Target | Concentration | Brand | Cat# |
|---|---|---|---|
HSP60 | 1:1000 | Abcam | Ab46798 |
ATP5A1 | 1:1000 | Invitrogen | 459240 |
γH2AX | 1:2500 | Abcam | Ab81299 |
53BP1 | 1:2500 | Abcam | Ab172580 |
Phalloidin 647 | 1:1000 | Abcam | Ab176759 |
Hoescht 33342 | 1 µg/ml | Thermo Scientific | 62249 |
Anti-rabbit | 1:1000 | Abcam | Ab150077 |
Anti-mouse | 1:1000 | Abcam | Ab150113 |
