Fig. 4: KDM1A targeting impairs CRC cells migration and invasion. | Cell Death Discovery

Fig. 4: KDM1A targeting impairs CRC cells migration and invasion.

From: Targeting lysine-specific demethylase 1 (KDM1A/LSD1) impairs colorectal cancer tumorigenesis by affecting cancer cells stemness, motility, and differentiation

Fig. 4

Timeline of the investigation of the effect of KDM1A targeting on invasion and migration (A). Representative phase-contrast pictures of wound healing assays of HCT116 and SW480 cells (B). Data analysis of the migration potential of adherent KDM1A-silenced cells vs control cells (shNT). Data represent the mean ± SD of at three independent experiments (C). Representative phase-contrast pictures of wound healing assays of CRC-SC#1 and CRC-SC#2 (D). Data analysis of the migration potential of KDM1A-silenced CRC-SCs vs controls (shNT). Data represent the mean ± SD of at least three independent experiments (E). Representative phase-contrast pictures of transwell invasion assay in adherent CRC cells stained with DiffQuick (F). Data analysis of invading cells at 48 h. Data represent the mean ± SD of at least three independent experiments (G). Wound healing assay of HCT116 cells treated with the KDM1A inhibitors ORY-1001 (10μmol/L) and GSK2879552 (40 μmol/L); representative pictures (H) and data analysis (I) of the migration potential of adherent cells treated with KDM1A inhibitors vs vehicle. Data represent the mean ± SD of at least three independent experiments. The percentage of wound area was assessed by ImageJ software. Student’s t-test was conducted (sh68 or sh71 versus shNT): *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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