Fig. 6: Proteomic analysis of CRC-SCs-silenced cells discloses alterations in RNA metabolism, mitochondrial function and cytoskeleton remodeling. | Cell Death Discovery

Fig. 6: Proteomic analysis of CRC-SCs-silenced cells discloses alterations in RNA metabolism, mitochondrial function and cytoskeleton remodeling.

From: Targeting lysine-specific demethylase 1 (KDM1A/LSD1) impairs colorectal cancer tumorigenesis by affecting cancer cells stemness, motility, and differentiation

Fig. 6

Semantic plot of the enriched biological processes (A). Proteomic validation through western blot of NUP21, filamin, villin, and SUCLG2 representative images (B) and quantification in CRC-SCs (C) Data represent the mean ± SD of at least 3 independent experiments. Representative images of transduced CRC-SC#1 and CRC-SC#2 stained with topro (blue) and in red filamin (D), LIMA (E), villin, and CDH17 (G) (scale bar 25 μm), IF quantification (F, H). Data represent the mean of at least 3 independent experiments ± SD. Student’s t-test was conducted (sh71 versus shNT): ***p < 0.001; ****p < 0.0001.

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