Fig. 1: ELK3 depletion enhances the sensitivity of TNBC cells to CDDP. | Cell Death Discovery

Fig. 1: ELK3 depletion enhances the sensitivity of TNBC cells to CDDP.

From: The ELK3-DRP1 axis determines the chemosensitivity of triple-negative breast cancer cells to CDDP by regulating mitochondrial dynamics

Fig. 1

A Representative images of Hs578T control and ELK3KD-578T cells treated with the indicated concentrations of CDDP for 48 h. Scale bar, 200 µm. B Relative proportions of ELK3KD-TNBC cells and control cells after 48 h of treatment with the indicated CDDP concentrations. C Representative flow cytometry plot (left) and bar graph (right) showing the proportions of ELK3KD-231 and ELK3KD-578T cells in different phases of the cell cycle after incubation with CDDP (5 µM) for 48 h. Statistical analysis compared the number of control and ELK3KD-TNBC cells at G2/M phase. D Measurement of intracellular ROS levels in MDA-MB231 control and ELK3KD-231 cells in the presence or absence of CDDP. E Flow cytometric plots (left) and bar graphs (right) showing the proportions of γ-H2AX-positive ELK3KD-TNBC and control cells after treatment with CDDP (5 µM) for 48 h. F Immunocytochemical imaging of γ-H2AX in MDA-MB231 control and ELK3KD-231 cells after CDDP treatment. Scale bar, 30 µm. All experiments were repeated at least three times and performed in triplicate. P values were calculated using a two-tailed Student’s t test (B–E). Data represent the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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