Fig. 2: CDDP induces mitochondrial ROS-dependent DNA damage in ELK3-depleted TNBC cells. | Cell Death Discovery

Fig. 2: CDDP induces mitochondrial ROS-dependent DNA damage in ELK3-depleted TNBC cells.

From: The ELK3-DRP1 axis determines the chemosensitivity of triple-negative breast cancer cells to CDDP by regulating mitochondrial dynamics

Fig. 2

A Flow cytometry histograms (left) and bar graphs (right) showing the results of MitoSOX staining for mitochondrial ROS in the presence or absence of the ROS scavenger N-acetyl-l-cysteine (NAC) and CDDP (5 µM). B Flow cytometry histograms (left) and bar graphs (right) showing the proportions of γ-H2AX-positive ELK3KD-TNBC and control cells after treatment with different combinations of NAC and CDDP for 48 h. C Relative proportions of ELK3KD-TNBC and control cells treated with CDDP in the presence or absence of NAC for 48 h. D Representative flow cytometry histogram (left) and bar graphs (right) showing the proportions of ELK3KD-TNBC and control cells in different phases of the cycle after incubation with CDDP in the presence or absence of NAC for 48 h. Cell cycling was measured using propidium iodide staining, and statistical analysis was performed by comparing the numbers of cells in G2/M phase. All experiments were repeated three times and performed in triplicate. P values were calculated using a two-tailed Student’s t test (A–D). Data represent the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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