Fig. 4: Negative correlation between YOD1 and miR-221/222 expressions and identification of a miR-221/222 target site at 3′ UTR of YOD1. | Cell Death Discovery

Fig. 4: Negative correlation between YOD1 and miR-221/222 expressions and identification of a miR-221/222 target site at 3′ UTR of YOD1.

From: miR-221/222 induce instability of p53 By downregulating deubiquitinase YOD1 in acute myeloid leukemia

Fig. 4

A Potential base pairing of YOD1 3′ UTR with miR-221/222-3p (red). B Expression of miR-221/222 in leukocytes from AML patients and healthy donors was detected using stem-loop RT-qPCR. (C) Negative correlation between YOD1 and miR-221/222 expression in peripheral blood leukocytes from patients with AML and healthy donors. Pearson’s correlation coefficients and p values are indicated. D U2OS cells were transfected with miRNA inhibitors or mimics, and YOD1 expression was analyzed via qPCR. E Schematic diagram of pmirGLO luciferase constructs containing the entire 3′ UTR of YOD1 (Entire), partial wild-type fragment (Target-WT), and partial mutant fragment (Target-MT). The sequence of miR-222-3p is shown. F Luciferase reporter constructs and miRNA mimics or inhibitor were used to transfect U2OS cells. Firefly luciferase (Fluc) and renal luciferase (Rluc) in the cell extracts were used to calculate the relative luciferase activity. p < 0.001 (**), p < 0.0001 (***) and p > 0.05 (ns). Representative results are shown from triplicate experiments with similar results.

Back to article page