Fig. 3: Ionizing radiation induces mitophagy in tumor cells.
From: Ionizing radiation triggers mitophagy to enhance DNA damage in cancer cells
Transmission electron microscopy (A) and immunofluorescence (B) analyses were used to examine the morphological changes in mitochondria of PANC-1 cells exposed to X-ray irradiation. Flow cytometry was used to quantify the amount of ROS (C) in mitochondria and cell acidification (D). Immunofluorescence assay revealed the co-localization of mitochondria and lysosomes in cells exposed to X-ray irradiation at 48 h (E). The expression of mitophagy-related proteins was quantified by western blot analyses (F–H). The TCGA database was used to compare Parkin expression levels versus pancreatic tumor survival rates (I). Parkin expression after X-ray irradiation in mouse melanoma (J) was detected by immunohistochemical staining.
