Fig. 1: SARS-CoV-2 solution injures HK2 cells and HUEhT cells. | Cell Death Discovery

Fig. 1: SARS-CoV-2 solution injures HK2 cells and HUEhT cells.

From: Inhibition of Toll-like receptor 4 and Interleukin-1 receptor prevent SARS-CoV-2 mediated kidney injury

Fig. 1

A Abnormal cell morphologies in HK2 and HUEhT cells were detected at a 1:106 and 1:101 dilution of SARS-CoV-2 solution 72 h after co-culture. Yellow arrowheads show cells with morphological abnormalities. The scale bar is 250 μm. B The representative images of TUNEL assay in HK2 and HUEhT cells incubated with SARS-CoV-2 solutions with different dilution ratios. SARS-CoV-2 significantly increased cell death of HK2 cells from culture 24–72 h in a time- and dose-dependent manner. A SARS-CoV-2 with low dilution rate induced cell death of HUEhT 72 h after co-culture. The scale bar is 200 μm. C The positive area of TUNEL staining was quantified using the ImageJ software and data are presented as mean ± SEM (each group; n = 3). One-way ANOVA with Dunnett’s multiple comparisons tests (control; Mock) was performed for statistical analyses, and significance was defined as **P < 0.01, ***P < 0.001.

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