Fig. 3: Paip1 depletion induces increased proteotoxic stress and activation of the integrated stress response pathway. | Cell Death Discovery

Fig. 3: Paip1 depletion induces increased proteotoxic stress and activation of the integrated stress response pathway.

From: Loss of Paip1 causes translation reduction and induces apoptotic cell death through ISR activation and Xrp1

Fig. 3

A, B” Wing imaginal discs from third instar larvae of control (A–A”) and Paip1-RNAi (B–B”) stained for Ubiquitin (red), GFP (green), and DAPI (blue). C, D” Wing imaginal discs from third instar larvae of control (C–C”) and Paip1-RNAi (D–D”) stained for Ref(2) P (red), GFP (green), and DAPI (blue). E, F” Wing imaginal discs from third instar larvae of control (E–E”) and Paip1-RNAi (F–F”) stained for ProteoStat (red), GFP (green), and DAPI (blue). G, H” Wing imaginal discs from third instar larvae of control (G–G”) and Paip1-RNAi (H–H”) stained for p-eIF2α (red), GFP (green) and DAPI (blue). I Statistical data of Ubiquitin level in (A–B”). J Statistical data of Ref(2) P level in (C–D”). K Statistical data of ProteoStat (ProteoStat area/Disc area) in (E–F”). L Statistical data of p-eIF2α level in (G–H”). For (A–H”), scale bars, 100 µm. For (I–L), data are mean ± SEM. n = 10 discs per genotype. Statistical analysis was performed using a two-tailed unpaired t-test. ****P < 0.0001.

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