Fig. 7: Knockdown of Paip1 upregulates Xrp1, which contributes to apoptotic cell death and ISR activation. | Cell Death Discovery

Fig. 7: Knockdown of Paip1 upregulates Xrp1, which contributes to apoptotic cell death and ISR activation.

From: Loss of Paip1 causes translation reduction and induces apoptotic cell death through ISR activation and Xrp1

Fig. 7

A Increased Xrp1 expression in the posterior compartment of wing discs expressing Paip1-RNAi. B–E” Xrp1 mediates cell death induced by Paip1 knockdown. Wing imaginal discs from third instar larvae of control (B–B”), Paip1-RNAi (C–C”), Xrp1-RNAi (D–D”) and Paip1-Xrp1 double RNAi (E–E”) stained for Dcp1 (red), GFP (green) and DAPI (blue). F Statistical data of apoptotic cell death (Dcp1 area/GFP area) in (E–H”). G–J” Xrp1 mediates phosphorylation of eIF2α in Paip1 knockdown wing discs. Wing imaginal discs from third instar larvae of control (G–G”), Paip1-RNAi (H–H”), Xrp1-RNAi (I–I”) and Paip1-Xrp1 double RNAi (J–J”) stained for p-eIF2α (red), GFP (green) and DAPI (blue). Higher level of phosphorylated eIF2α upon Paip1 knockdown (H–H”, labeled with GFP) was reduced by knocking down Xrp1 simultaneously (J–J”). K Statistical data of p-eIF2α level in (J–M”). For (A), scale bars, 100 µm. For (B–E”) and (G–J”), scale bars, 100 µm. For (F) and (K), data are mean ± SEM. n = 10 discs per genotype. Statistical analysis was performed using a two-tailed unpaired t-test. ****P < 0.0001.

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