Fig. 8: Loss of Paip1 results in an increase in Xrp1 translation mediated by its 5’UTR. | Cell Death Discovery

Fig. 8: Loss of Paip1 results in an increase in Xrp1 translation mediated by its 5’UTR.

From: Loss of Paip1 causes translation reduction and induces apoptotic cell death through ISR activation and Xrp1

Fig. 8

A Schematic of structure of the 5’UTR of the Drosophila Xrp1 mRNA. There are two predicted uORFs in the 5’UTR, and the uORF2 overlaps with the main CDS region in a different reading frame. B Relative ribosomal occupancy on Xrp1 mRNA. C Schematic of luciferase-reporters for control, wild-type Xrp1 5’UTR and mutated Xrp1 5’UTR. D Western blot analysis of Paip1 dsRNA transfection efficiency. Western blot of control and Paip1 knockdown cell lysate probed with anti-Paip1 and anti-Actin antibodies. Quantification was shown in the barplot. Data are mean ± SEM, n = 3. Statistical analysis was performed using a two-tailed unpaired t-test. ****P < 0.0001. E Effect of Paip1-depletion on the luciferase reporter activity (psi-check2 control plasmid, Xrp1-UTR-reporter, and Xrp1-mUTRUAA-reporter). Relative luciferase activity was measured by Renilla activity/Firefly activity and normalized to NC-psi-check2. Data are mean ± SEM, n = 3. Statistical analysis was performed using a two-tailed unpaired t-test. ****P < 0.0001; *P < 0.05; ns, P > 0.05. F Working model describing the roles of ISR and Xrp1 on apoptosis in Paip1 depletion cells.

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