Fig. 3: NeuroD4-induced neuronal reprogramming suppresses the proliferative marker EdU and Ki67 in glioblastoma cells. | Cell Death Discovery

Fig. 3: NeuroD4-induced neuronal reprogramming suppresses the proliferative marker EdU and Ki67 in glioblastoma cells.

From: NeuroD4 converts glioblastoma cells into neuron-like cells through the SLC7A11-GSH-GPX4 antioxidant axis

Fig. 3

A The experimental design for labeling EdU in glioblastoma cells. B–E EdU detection of U251 and KNS89 cells at 14 dpi and quantitative analysis of EdU+ (9 random fields from triplicate samples were captured for quantification). F–I Immunocytochemical analysis of the Ki67 marker in U251 and KNS89 cells at 14 dpi and quantitative analysis of Ki67+ (9 random fields from triplicate samples were captured for quantification). J, K Time-course cell counting at 0, 1, 3, 5, 7, 14, and 21 dpi. The data are presented as mean ± SD. ***P < 0.001. Dpi: days post infection; GM glioblastoma cell medium, NM neuronal induction medium. Scale: 100 µm.

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