Fig. 2: Sal-treated hepatocytes suppress HSC activation.

Primary HSCs were isolated from healthy mice. A Protein expressions and (B) mRNA expressions of α-SMA, Col1A1 and FN in HSCs at day 1 and day 3. C The quantitative results of EdU assay of cell proliferation in HSCs at day 1 and day 3. D EdU assay of cell proliferation in HSCs at day 1 and day 3. E Illustration of the indirect co-culture system: HSCs co-cultured with Sal-treated hepatocytes, using Figdraw. F Protein expressions and (G) mRNA expressions of α-SMA, Col1A1 and FN in HSCs co-cultured with Sal-treated hepatocytes. H EdU assay of cell proliferation in HSCs co-cultured with Sal-treated hepatocytes. Each value is the mean ± SD of three experiments. **p < 0.01, ***p < 0.001.