Fig. 2: ANXA7 is significantly downregulated after neuron injury. | Cell Death Discovery

Fig. 2: ANXA7 is significantly downregulated after neuron injury.

From: Targeting ANXA7/LAMP5-mTOR axis attenuates spinal cord injury by inhibiting neuronal apoptosis via enhancing autophagy in mice

Fig. 2

a Neurons were treated with OGD/R, after which immunofluorescence staining was used to detect ANXA7 protein level. Scale bars: 10 µm. b Neurons were treated with OGD/R and Western blotting was used for protein detection. c Spinal cord injury model was performed in C57BL/6 mice, the distribution of ANXA7 were detected by immunohistochemistry staining in head-end, tail-end and injury center in spinal cord after 24 h of injury. Scale bars: 200 µm. d ANXA7 mRNA level in mice after 0 h, 6 h, 12 h, 24 h, 3 d and 5 d of spinal cord injury. e, f Protein level of ANXA7 in neuron after 24 h of spinal cord injury detected by immunohistochemistry staining. g–i Neurons were treated with 0.1% DMSO, SEC or ABO separately 1 h before OGD/R treatment, and CCK-8 assay was used to detect survival ratio of neurons. Data are presented as means ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, n = 3.

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