Fig. 6: Asp411 residue of ANXA7 is an essential site for maintaining the interaction between ANXA7 and LAMP5. | Cell Death Discovery

Fig. 6: Asp411 residue of ANXA7 is an essential site for maintaining the interaction between ANXA7 and LAMP5.

From: Targeting ANXA7/LAMP5-mTOR axis attenuates spinal cord injury by inhibiting neuronal apoptosis via enhancing autophagy in mice

Fig. 6

a Structure of interaction of ANXA7 and LAMP5, and the predictive results of amino acid sites regulating the interaction between ANXA7 and LAMP5. b Schematic representation of the mutation site in plasmid. c, d LAMP5 antibody was used in co-immunoprecipitation to detect the interaction between ANXA7 and LAMP5 after transfected with ANXA7 overexpression plasmid, ANXA7D452A plasmid, ANXA7D411A plasmid and ANXA7D411A/D452A plasmid. e–g Western blot was used to detect protein level of LC3B and c -Caspase3 in neurons transfected with ANXA7 overexpression plasmid and ANXA7D411A plasmid after OGD/R. Data are presented as means ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, n = 3.

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