Fig. 1: Transcriptome analysis of differentiated hiPSCs (IMR90) toward germ layer cells. | Cell Death Discovery

Fig. 1: Transcriptome analysis of differentiated hiPSCs (IMR90) toward germ layer cells.

From: Transcriptome-based prediction of drugs, inhibiting cardiomyogenesis in human induced pluripotent stem cells

Fig. 1

A Overview of the UKK2-CTT for the in vitro cardiac differentiation of the experimental design from day −2 to day14. B To determine the the early germ layer formation, hiPSCs were exposure to 1 hr, 24 h (day1) and 48 h CHIR (day2) compared with those of untreated controls. The hiPSCs were cultured as a monolayer on matrigel-coated plates for 2 days under pluripotent conditions and on day 0 exposed to GSK3 inhibitor, CHIR (10 µM) for 24 h. After microarray analysis of the RNA, the number of the up- and down regulated SPSs (log2 fold change > 1; adjusted p‐value < 0.05) were determined. C Visualization of enriched gene ontology terms across IMR90 1 h, 24 h and 48 h after CHIR. Heatmap showing the top 20 enrichment clusters, colored by p-values. D Mesendoderm gene table Representative mesendoderm genes increased gradually for the different time points, after exposure to CHIR in IMR90-hiPSCs.

Back to article page