Fig. 2: TIALD inhibits HCC metastasis both in vitro and in vivo. | Cell Death Discovery

Fig. 2: TIALD inhibits HCC metastasis both in vitro and in vivo.

From: LncRNA TIALD contributes to hepatocellular carcinoma metastasis via inducing AURKA lysosomal degradation

Fig. 2

A Relative expression of TIALD in LO2 and different liver cancer cell lines. B Expressions of TIALD in SNU449 and SK-Hep-1 cells stably transfected with TIALD or Control and in SMMC-7721 cells stably transfected with shTIALD or shNC were detected by real-time qRT-PCR. C Representative and quantification results of transwell cell migration and invasion assay in SMMC-7721 and SNU449 cells. D Representative and quantification results of wound healing assay. E Western blotting detection of epithelial (E-cadherin), mesenchymal (N-cadherin) and transcription factors (Slug and Snail) in SMMC-7721 and SNU449 cells. F Representative images and quantification data of metastatic nodules in the lung tissues of mice. Stable cell lines of TIALD over-expression or knockdown were injected by tail vein in B-NDG mice. G Representative images of lung metastatic nodules stained with H&E. H Representative images of IHC staining in lung metastasis nodules of mice.

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