Fig. 3: METTL16 mediated downregulation of TIALD in HCC. | Cell Death Discovery

Fig. 3: METTL16 mediated downregulation of TIALD in HCC.

From: LncRNA TIALD contributes to hepatocellular carcinoma metastasis via inducing AURKA lysosomal degradation

Fig. 3

A LncRNA pulldown combined with mass spectrometry to Identify TIALD interaction proteins (left) and list of potential interacting proteins of TIALD (right). B RNA pull-down assay was performed in SMMC-7721 and SNU449 cells using biotin-labeled TIALD RNA probe transcribed in vitro, and METTL16 was detected using western blots. C RIP-qPCR analysis of the interaction of METTL16 and TIALD in SMMC-7721 and SUN449 cells. Enrichment of TIALD with METTL16 antibody and IgG control was measured by qPCR. D METTL16 was knock-down or over-expressed in SMMC-7721 and SNU449 cells, the expression of METTL16 was detected by western blots and the expression of TIALD was detected by qPCR. E FISH analysis of the expression of TIALD in METTL16 over-expression SMMC-7721 cells and METTL16 knockdown SNU449 cells. F The correlation between METTL16 and TIALD in TCGA datasets (up) and qRT-PCR analysis of METTL16 and TIALD in 33 HCC tissues (down). G Wild-type and different fragment of TIALD sequences were cloned into a luciferase reporter (up). Relative luciferase activity of the wild-type and different fragment of TIALD reporter vectors catalyzed by METTL16 (down).

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