Fig. 1: Preparation of human prostate cancer cells with KIF15 knockdown. | Cell Death Discovery

Fig. 1: Preparation of human prostate cancer cells with KIF15 knockdown.

From: Knockdown of KIF15 suppresses proliferation of prostate cancer cells and induces apoptosis through PI3K/Akt signaling pathway

Fig. 1

A Detection of KIF15 expression in normal tissues and prostate cancer tissues with different pathological stages by IHC. B The IHC scores of normal tissues and prostate cancer tissues were collected and compared. C The differential expression of KIF15 in prostate cancer tissues and normal tissues was analyzed based on the data collected from the TCGA-PRAD dataset. D The correlation between KIF15 expression and prostate cancer patients’ prognosis (progression-free survival and overall survival) was analyzed based on the data collected from the TCGA-PRAD dataset. E The endogenous expression of KIF15 in immortalized cells of human normal prostate matrix WPMY-1 and human prostate cancer cell lines, including PC-3, DU 145, and C4-2, was detected by qPCR and western blot analysis, respectively. F The screening of more efficient shRNA was completed by qPCR. G The fluorescence of cells, which were infected with shCtrl or shKIF15 for 72 h, observed by microscope demonstrates a >80% efficiency of infection. H&I, The knockdown efficiencies of KIF15 in DU 145 and PC-3 cells were detected by qPCR (H) and western blot analysis (I). Data were shown as Mean with SD. **P < 0.01, ***P < 0.001.

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