Fig. 3: Overexpression of KIF15 promoted prostate cancer development in vitro. | Cell Death Discovery

Fig. 3: Overexpression of KIF15 promoted prostate cancer development in vitro.

From: Knockdown of KIF15 suppresses proliferation of prostate cancer cells and induces apoptosis through PI3K/Akt signaling pathway

Fig. 3

The overexpression efficiencies of KIF15 in DU 145 and PC-3 cells were detected by qPCR (A) and western blot analysis (B). C CCK8 assay was used to detect cell proliferation of DU 145 and PC-3 cells in Control and KIF15 groups with time from 24 h to 120 h. D After lentivirus infection of DU 145 cells and PC-3 cells, the number of colonies of the KIF15 group and Control group was counted and compared during 7 days of culturing. E 5 days after lentivirus infection, the apoptotic cell percentage of the KIF15 group and Control group was detected by flow cytometry. F Cell motility of DU 145 and PC-3 cells with or without KIF15 overexpression was detected by Transwell assay. Moreover, KIF15 knockdown cell models were transfected with KIF15 overexpression lentivirus for recovering KIF15 expression, which was subjected to the detection of cell proliferation (G) and cell apoptosis (H). Data were shown as Mean with SD. **P < 0.01, ***P < 0.001.

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