Fig. 1: Nat10 haploinsufficiency delays wound repair.
From: N-acetyltransferase 10 promotes cutaneous wound repair via the NF-κB-IL-6 axis
A Representative images showing the wound healing process of Nat10+/− mice (n = 7) and their WT littermates (n = 10). Yellow arrows indicate the evident delayed wound repair in Nat10+/− mice. Scale bars, 5 mm. B Wound closure of (A) was quantified and unhealed rate was presented as the percentage of wound compared with the initial wound area size. n.s., no significance. C H&E staining of the wounded skin tissues derived from WT and Nat10+/− mice on day 5 after punching. Black lines, unhealed wound; Arrows, fresh skin with re-epithelialization. Scale bars, 100 μm. D The chart showing quantified wound width basing on the H&E staining images (WT: n = 9, Nat10+/−: n = 11). E Quantification of the re-epithelialization in wounds of both genotypes based on the H&E staining images (WT: n = 9, Nat10+/−: n = 11). F qPCR analysis of Nat10, Fn1 and Acta2 expression in wounded skin tissues collected from WT and Nat10+/− mice, n = 3 for each genotype. Immunoblot analysis of NAT10, FN1 and α-SMA protein expression in wounded skin tissues of WT and Nat10+/− mice at day 5 after wounding (G), and the relative quantification were presented in (H) using ImageJ Plus 6.0 (n = 3 mice for each group). The data are presented as the means ± SEM, * P < 0.05, ** P < 0.01, *** P < 0.001, determined by Student’s t test.
