Fig. 2: NAT10 is crucial for keratinocyte migration.
From: N-acetyltransferase 10 promotes cutaneous wound repair via the NF-κB-IL-6 axis
A Single cell RNA-sequencing analysis showing the expression of Nat10 in various skin cell types. Original data were from public online tool of The Human Protein Atlas. B, C Immunoblot and qPCR analysis showing NAT10 protein and mRNA levels in HaCaT cells with or without knocking down NAT10 expression after 72 h of transfection (n = 3 for each group). Representative images (D) and related quantification (E) showing the migrated cells with or without NAT10 knockdown by using trans-well assay for 24 h. Scale bars, 50 μm. F, G Representative images (F) and related quantitative analysis showing the wound closure of HaCaT cells treated with or without NAT10 siRNA by using wound healing assays for 24 h (n = 6 for each group). Scale bars, 50 μm. H qPCR analysis of MMP2 expression in HaCaT cells with indicated treatments after scratching for two hours (n = 3 for each group). I Immunoblotting analysis of p-FAK (Y397) in HaCaT cells with or without NAT10 knockdown after scratching. Data are presented as the means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, obtained by Student’s t test.
