Fig. 5: NAT10 maintains nuclear p65 stability via restraining its poly-ubiquitination.
From: N-acetyltransferase 10 promotes cutaneous wound repair via the NF-κB-IL-6 axis
A Immunoblotting analysis of cytoplasmic and nuclear p65 activation in HaCaT cells with or without NAT10 siRNAs and TNFα treatment. GAPDH and Lamin B1 marked the cytoplasmic and nuclear protein fraction, respectively. N, nucleus; C, cytoplasm. B, C The relative levels of nuclear p65/Lamin B1 and p-p65/Lamin B1 in (A) were quantified from 3 independent experiments. D, E Immunoblotting (D) showing nuclear p65 levels in Leptomycin B-treated HaCaT cells with control or NAT10 siRNA treatment. The relative level of p65/Lamin B1 was quantified in (E) (n = 3 for each group). F Immunoblotting showing the poly-ubiquitination of nuclear p65 in HaCaT cells with or without NAT10 knockdown. Data are presented as the means ± SEM. * P < 0.05, ** P < 0.01, determined by Student’s t test.
