Table 1 The sequences of RNA and DNA oligonucleotides.
Name | Sense Strand/Sense Primer (5’-3’) | Sense Strand/Sense Primer (5’-3’) |
|---|---|---|
siRNA duplexes | ||
siPGC-1α | GCAAUAAAGCGAAGAGUAUTT | AUACUCUUCGCUUUAUUGCTT |
siPGC-1α | CCACCACUCCUCCUCAUAATT | UUAUGAGGAGGAGUGGUGGTT |
siPGC-1α | GCUACCUGAGAGAGACUUUTT | AAAGUCUCUCUCAGGUAGCTT |
NC | UUCUCCGAACGUGUCACGUTT | ACGUGACACGUUCGGAGAATT |
Primers for qPCR | ||
PGC-1α (mouse) | TATGGAGTGACATAGAGTGTGCT | GTCGCTACACCACTTCAATCC |
PGC-1α (human) | TGGTGCCACCATCAAAGA | TCACCAAACAGCCGCAGACTG |
Sod1 (mouse) | GCCCGGCGGATGAAGA | CGTCCTTTCCAGCAGTCACA |
Sod3 (mouse) | CAGACAAAGGAGCGCAAGAAG | TGAGGCTTAAGTGGTCTTGCA |
Mrc-1 (mouse) | GGGACGTTTCGGTGGACTGTGG | TTGTGGGCTCTGGTGGGCGA |
Arg1 (mouse) | GATGTCCCTAATGACAGCTCC | AGCACCACACTGACTCTTCC |
Ym-1 (mouse) | CCCCAGGAAGTACCCTATGCCT | AACCACTGAAGTCATCCATGTCC |
Collagen I (mouse) | GCT CCT CTT AGG GGC CAC T | CCA CGT CTC ACC ATT GGG G |
α-SMA (mouse) | GTCCCAGACATCAGGGAGTAA | TCGGATACTTCAGCGTCAGGA |
TGF-β (mouse) | CTCCCGTGGCTTCTAGTGC | GCCTTAGTTTGGACAGGATCTG |
TNF-α (mouse) | CATCTTCTCAAAATTCGAGTGACAA | TGGGAGTAGACAAGGTACAACCC |
IL-1β (mouse) | CCGTGGACCTTCCAGGATGA | GGGAACGTCACACACCAGCA |
IL6 (mouse) | AGTTGCCTTCTTGGGACTGA | TCCACGATTTCCCAGAGAAC |
MCP1 (mouse) | TAAAAACCTGGATCGGAACCAAA | GCATTAGCTTCAGATTTACGGGT |
IL-10 (mouse) | CCAAGCCTTATCGGAAATGA | TTTTCACAGGGGAGAAATCG |
HGF (mouse) | AAAGGGACGGTATCCATCACT | GCGATAGCTCGAAGGCAAAAAG |
STAT3 (mouse) | CACCTTGGATTGAGAGTCAAGAC | AGGAATCGGCTATATTGCTGGT |
GAPDH (mouse) | ACCACAGTCCATGCCATCAC | ACGGATACATTGGGGGTAGG |
Nrf2 (mouse) | GGTTGCCCACATTCCCAAAC | TCCTGCCAAACTTGCTCCAT |
GAPDH (human) | GAGTCAACGGATTTGGTCGT | GACAAGCTTCCCGTTCTCAG |
