Fig. 1: Nuclear size is associated with cell fate decision and chromatin-modifying enzyme activity.
From: Chromatin-modifying enzymes as modulators of nuclear size during lineage differentiation
A Immunofluorescence staining cell nucleus of hESCs, hESC-derived NPCs, hESC-derived iN cells by DAPI (blue). White scale bars = 50 μm. B Measurements of cell nucleus size of hESCs, hESC-derived NPCs, hESC-derived iN cells by DAPI staining. Values represent mean ± SD (n = 3). ***P < 0.001. C Brightfield and fluorescent images of hESCs and hESC-derived NPCs immunofluorescence staining for H3K9me3 (red) and DAPI (blue). D Measurements of nuclear size after hESCs treated with histone demethylase inhibitors (GSK-J1, (R)-2-Hydroxyglutaric acid disodium salt, GSK-LSD1, NSC636819, PBIT, IOX1, JIB04), DNA methyltransferase inhibitor (5-Azacytidine), and histone acetyltransferase inhibitor (C646) by DAPI staining. Values represent mean ± SD (n = 3). Statistically significant differences are marked with asterisks. *P < 0.05, ***P < 0.001. E Representative immunocytochemistry images of hESCs stained with DAPI. hESCs treated with DMSO as control and 5-Aza. It was shown that the bigger nuclei occurred at 5-Aza treatment. The images were taken at 40× on a confocal microscope. White scale bars = 20 μm.
