Fig. 4: Rapamycin reversed the effects of Ang-(1-7) in LPS-induced human bronchial epithelial cells. | Cell Death Discovery

Fig. 4: Rapamycin reversed the effects of Ang-(1-7) in LPS-induced human bronchial epithelial cells.

From: Exogenous Ang-(1-7) inhibits autophagy via HIF-1α/THBS1/BECN1 axis to alleviate chronic intermittent hypoxia-enhanced airway remodelling of asthma

Fig. 4

A Immunofluorescence results of α-SMA in human bronchial epithelial cells with CIH, Ang-(1-7) or Ang-(1-7) along with RAPA. B Quantitative analysis of α-SMA. C Immunofluorescence results of E-cadherin. D Quantitative analysis of E-cadherin. E Immunofluorescence results of Collagen IV. F Quantitative analysis of Collagen IV. G Western blot results of E-cadherin, Vimentin and Snail. H Western blot results of α-SMA and Collagen IV. I Western blot results of LC3B and p62. J Representative images of immunofluorescence staining of mRFP-GFP-LC3 in human bronchial epithelial cells. Representative profiles of autophagosomes (RFP + GFP + dots) and autolysosomes (RFP + GFP − dots). K Quantitative analysis of mRFP-GFP-LC3. Data are expressed as mean ± SD (n = 3).

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