Fig. 5: Ang-(1-7) inhibited EMT and fibrosis in LPS-induced human bronchial epithelial cells via HIF-1α/THBS1 axis. | Cell Death Discovery

Fig. 5: Ang-(1-7) inhibited EMT and fibrosis in LPS-induced human bronchial epithelial cells via HIF-1α/THBS1 axis.

From: Exogenous Ang-(1-7) inhibits autophagy via HIF-1α/THBS1/BECN1 axis to alleviate chronic intermittent hypoxia-enhanced airway remodelling of asthma

Fig. 5

A Volcano plot of differential expressed genes. B qRT-PCR results of THBS1 in mice lung tissues. C Immunofluorescence results of THBS1 in mice with OVA or OVA + CIH or OVA + CIH + Ang-(1-7) and quantitative analysis of THBS1. D Western blot results of HIF-1α and THBS1 in mice lung tissues. E qRT-PCR results of THBS1 in human bronchial epithelial cells. F Western blot results of HIF-1α and THBS1 in human bronchial epithelial cells. G Jaspar predicts a putative HIF-1α binding site locating on the promoter region of THBS1. H ChIP assay of the relative enrichment of HIF-1α on the promoter region of THBS1. I qRT-PCR results of THBS1 in human bronchial epithelial cells infected with adenovirus overexpressing THBS1 (ad-THBS1). J Western blot results of THBS1 in human bronchial epithelial cells. K Immunofluorescence results of α-SMA in human bronchial epithelial cells with CIH, Ang-(1-7) or Ang-(1-7) infected with ad-THBS1. L Immunofluorescence results of E-cadherin and quantitative analysis of E-cadherin. M Immunofluorescence results of Collagen IV and quantitative analysis of Collagen IV. N Western blot results of E-cadherin, Vimentin and Snail. O Western blot results of α-SMA and Collagen IV. Data are expressed as mean ± SD (n = 3).

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