Fig. 6: Ang-(1-7) inhibited autophagy in LPS-induced human bronchial epithelial cells via HIF-1α/THBS1 axis. | Cell Death Discovery

Fig. 6: Ang-(1-7) inhibited autophagy in LPS-induced human bronchial epithelial cells via HIF-1α/THBS1 axis.

From: Exogenous Ang-(1-7) inhibits autophagy via HIF-1α/THBS1/BECN1 axis to alleviate chronic intermittent hypoxia-enhanced airway remodelling of asthma

Fig. 6

A Representative images of immunofluorescence staining of mRFP-GFP-LC3 in human bronchial epithelial cells. Representative profiles of autophagosomes (RFP + GFP + dots) and autolysosomes (RFP + GFP − dots). B Quantitative analysis of mRFP-GFP-LC3. C Western blot results of LC3B and p62. D Interaction between THBS1 and BECN1-PIK3C3 detected by immunoprecipitation (IP) analysis in human bronchial epithelial cells with LPS/CIH or Ang-(1-7). E Interaction between THBS1 and BECN1-PIK3C3 detected by IP analysis in human bronchial epithelial cells. F Duolink PLA in situ assays in human bronchial epithelial cells. G Interaction between THBS1 and ULK1/ULK2 detected by IP analysis in human bronchial epithelial cells. Data are expressed as mean ± SD (n = 3).

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