Fig. 2: Effect of reduced IDO1 expression on EC cell phenotypes.
From: IDO1 facilitates esophageal carcinoma progression by driving the direct binding of NF-κB and CXCL10
A The relative mRNA expression of IDO1 in human EC cell lines and normal esophageal epithelial cells HEEC was evaluated by qRT-PCR. B The silencing efficiency of IDO1 was detected by qRT-PCR after the small hairpin RNA (shRNA) interferes with the expression of IDO1 in TE-1 and ECA-109 cells. C After shRNA interfered with IDO1 expression in EC cells, its protein expression was detected by western blotting. D, E Effect of IDO1 expression reduction on (D) clone formation and (E) proliferation in TE-1 and ECA-109 cells. F The effect of decreased expression of IDO1 in EC cells on their apoptosis was detected by flow cytometry. G, H EC cells with reduced IDO1 expression underwent (G) Transwell experiments and (H) wound healing experiments to assess migration, respectively. The data are represented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.
